Which statement about restriction enzymes is incorrect?

Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ

Published on: August 12, 2026

Restriction enzymes cut DNA at specific sequence called recognition sites.

A restriction enzyme always cut DNA to leave the same sequence at the ends.

Some restriction enzymes cut the two DNA strands at slightly different points within their recognition site to make a ‘sticky’ end.

Restriction enzymes are exonucleases rather than endonucleases.

Text Solution

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The correct answer is:

This statement is incorrect because restriction enzymes are endonucleases, not exonucleases. They cut DNA at specific internal recognition sites.

Correct → they cut at specific recognition sequences

Correct → they produce specific ends (sticky or blunt depending on enzyme)

Correct → many cut DNA strands at staggered positions to form sticky ends

Incorrect → they are endonucleases, not exonucleases

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